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TOPAZ® User Documentation

Customers may obtain the following materials through technical support.

Workbooks

Translation Workbook
MS Excel-based workbook used to help design translation grids.

Optimization Workbook
MS Excel-based workbook used to help design in-chip optimization grids.

Technical Notes

Co-Crystallization Technical Note
This Technical Note describes protocols that can be used with TOPAZ® screening chips to identify crystallization conditions for novel protein-ligand complexes.

Quick Reference Guides

TOPAZ® Screening Chips

1.96 Quick Reference Card
Simplifies the list of steps to prepare the 1.96 Chip for a crystallization screen and displays the numbering system for mapping the reagent inlets and diffusion chambers on the 1.96 Chip.

4.96 Quick Reference Card
Shows the features of the 4.96 Chip and explains how to use the cell numbers on the chip to locate each diffusion experiment.

8.96 Quick Reference Card
Shows the features of the 8.96 Chip and explains how to use the cell numbers on the chip to locate each diffusion experiment.

User Guides

Translation Guide
Describes how to optimize hits using TOPAZ® screening chips and translate hits to crystals that are of sufficient size and quality to collect X-ray diffraction data.

TOPAZ® Screening Chips

1.96 Screening Chip User Guide
Describes how to set up a crystallization screen of 96 diffusion experiments (1 sample vs. 96 reagents) on the 1.96 Chip, with the aid of the TOPAZ™ FID Crystallizer.

4.96 Screening Chip User Guide
Describes how to set up a crystallization screen of 384 diffusion experiments (4 samples vs. 96 reagents) on the 4.96 Chip, with the aid of the TOPAZ® FID Crystallizer.

TOPAZ® FID Crystallizer

FID Crystallizer User Guide
Describes how to install, operate and maintain the FID Crystallizer.

Product Inserts

TOPAZ® OptiMix™ Reagents

  • OptiMix Formulations Sheet
  • OptiMix-1 Plate Kit
  • OptiMix-1 Tube Set
    OptiMix-2 Plate Kit
  • OptiMix-2 Tube Set
    OptiMix-3 Plate Kit
  • OptiMix-3 Tube Set
    OptiMix-4 PEG Plate Kit
  • OptiMix-4 PEG Tube Set
    OptiMix-5 Membrane Plate Kit
  • OptiMix-5 Membrane Tube Set

Material Safety Data Sheets (MSDS)

  • Fluidigm Hydration Fluid Concentrate
  • Catalase Install Kit
  • OptiMix-1 Reagents
  • OptiMix-2 Reagents
  • OptiMix-3 Reagents
  • OptiMix-PEG Reagents
  • OptiMix-5 Reagents
  • OptiMix Stock-Salts
  • OptiMix Stock-Buffers
  • OptiMix Stock-Precipitants